Journal of Applied Entomology (2007) 131, 548-552

Xian Feng, Nai-Zhong Chen, Jun Ma, Shui-Fang Zhu and Xue-Nan Hu (2007)
Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real-time PCR
Journal of Applied Entomology 131 (8), 548-552
Abstract: Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L. trifolii based on real-time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L. trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L. trifolii-specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real-time PCR assay system was 2 h, and it would save 3-7 h compared with conventional PCR.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jun Ma

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liriomyza trifolii		Germany
	Liriomyza trifolii		China (south)
	Liriomyza trifolii		Japan
	Liriomyza trifolii		Philippines
	Liriomyza trifolii		Taiwan
	Liriomyza trifolii		Israel
	Liriomyza trifolii		Honduras
	Liriomyza trifolii		Mexico